Author: oetltd

We are baculovirus expression specialists. Providing support and services in an ever changing research environment.

Baculovirus P10 Structures and Role in Insect Cells

A blog on baculovirus P10 structures and the role in insect cells might appear a slightly odd topic for a blog from a company based on expression of recombinant proteins. However, a recently published paper that is the product of a collaboration between the Insect Virus Research Group at Oxford Brookes University and OET Ltd offers some insight to the role of P10 structures in infected insect cells and why our flashBAC ULTRA vector is so successful (Graves et al., 2019). flashBAC ULTRA, like all other versions of this expression vector, offers a one-step process for making recombinant viruses but lacks several of the baculovirus genes, including p10. (more…)

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New pOET9 Vectors!

This month we’re celebrating the release of our newest product to come out of the OET labs – pOET9 transfer vectors. With the application of baculoviruses in mammalian protein expression becoming increasingly popular it seemed only right to introduce a new set of transfer vectors that will facilitate the production of recombinant proteins in mammalian cell lines using Autographa californica nucleopolyhedrovirus. (more…)

Plaque-Assay Schematic

We’ve said it enough times; the plaque-assay is the ‘gold standard’ for determining virus infectivity. But this doesn’t make the procedure any easier! Whether it’s the agonising 5 day waiting period or the disorientating number of steps, plaque-assays can seem like a chore and we’ve heard of more than enough non-virologists admit to skipping this laborious technique in favour of a simpler solution. (more…)

How to Grow Cells over the Holiday Period

We want to talk about how to grow cells over the holiday period spanning Christmas and New Year.  (OK, so we have covered this before but we think it is worth repetition for new users of the baculovirus-insect cell system).  Most labs, academic and industry, will have some time off over the next few weeks. It is often a problem keeping your cell cultures in good condition so that when you return in 2019 you can pick up that important protein expression project without too much delay.  Given that most insect cells (usually Spodoptera frugiperda or Trichoplusia ni cell lines) need to be sub cultured at least twice a week if they are grown as suspension cultures, how will they survive for this protracted period? (more…)

Growing Mammalian Cells Successfully

While OET Ltd has built its reputation on baculovirus-insect cell expression systems, we also undertake work that requires growing mammalian cells successfully too.  Much of this work involves the transduction of these cells with BacMam vectors to express genes placed under the control of mammalian-specific promoter elements.  Some of our work in this area was recently published and involved the generation of improved BacMam vectors for transducing both mammalian cells in culture and also human pancreatic islet cells for pre-clinical tests of gene therapy.  (more…)

Growing insect cells successfully – Part II

In our previous blog we talked about the early stages of growing insect cells when you might just have revived a frozen stock and set up either monolayer or suspension cultures (or both).  If you have established a viable suspension culture – and here we are talking primarily about insect cells such as Sf21 or Sf9 – you will probably monitor them fairly closely for their rate of growth.  The ideal scenario is that your cells will double in number every 24 hours.  However, frequently this isn’t the case.  Why not? (more…)

Growing Insect Cells Successfully

With Autumn, or Fall as some of you may call it, upon us everyone seems to back in action in the lab again and hopefully growing insect cells successfully.  Regular readers of these blogs will know that we frequently return to the question of how to propagate insect cells.  The reason for doing so is because growing insect cells successfully is fundamental to producing recombinant proteins using the baculovirus system. (more…)

Baculovirus Transfer Vector Plasmid Compatibility

A frequent customer query concerns baculovirus transfer vector plasmid compatibility with either flashBACTM or other systems for making recombinant viruses.  Obviously, if you are buying flashBACTM and our pOET range of transfer vectors you won’t have a problem.  However, given that baculovirus expression vectors have been around since 1983 and many labs have produced variations around a theme, it is not surprising that confusion can arise. (more…)

OET VISITS KEY PARTNERS IN MEXICO FOR DIABETES TYPE I RESEARCH

OET scientists, Dr Mine Aksular and Dr Leo Graves, recently travelled to Mexico City to spend six days visiting the Centre for Molecular and Cell-Based Therapeutics (CMCBT) as part of an international research collaboration (TRANSDIA) investigating a novel therapy for type I diabetes. Accompanied by collaborator Dr Stephen Hughes from the Nuffield Department of Surgical Sciences, Oxford University, the team were able to provide expert advice and practical guidance on key aspects of the project, helping to facilitate the transfer of technology to Mexican researchers. (more…)